Plasmid

Part:BBa_K3779009:Design

Designed by: JiaCheng, Li   Group: iGEM21_NWU-CHINA-B   (2021-06-21)


pPIC9K-SS-bgly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7794
    Illegal EcoRI site found at 13316
    Illegal EcoRI site found at 18563
    Illegal EcoRI site found at 27993
    Illegal XbaI site found at 14126
    Illegal XbaI site found at 19373
    Illegal XbaI site found at 28803
    Illegal PstI site found at 1920
    Illegal PstI site found at 3161
    Illegal PstI site found at 5716
    Illegal PstI site found at 7543
    Illegal PstI site found at 8683
    Illegal PstI site found at 11238
    Illegal PstI site found at 13065
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7794
    Illegal EcoRI site found at 13316
    Illegal EcoRI site found at 18563
    Illegal EcoRI site found at 27993
    Illegal NheI site found at 8365
    Illegal NheI site found at 13887
    Illegal NheI site found at 14834
    Illegal NheI site found at 19134
    Illegal NheI site found at 20081
    Illegal NheI site found at 28564
    Illegal NheI site found at 29511
    Illegal PstI site found at 1920
    Illegal PstI site found at 3161
    Illegal PstI site found at 5716
    Illegal PstI site found at 7543
    Illegal PstI site found at 8683
    Illegal PstI site found at 11238
    Illegal PstI site found at 13065
    Illegal NotI site found at 7806
    Illegal NotI site found at 13328
    Illegal NotI site found at 18575
    Illegal NotI site found at 28005
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7794
    Illegal EcoRI site found at 13316
    Illegal EcoRI site found at 18563
    Illegal EcoRI site found at 27993
    Illegal BglII site found at 4170
    Illegal BglII site found at 6573
    Illegal BglII site found at 9692
    Illegal BglII site found at 12095
    Illegal BglII site found at 17342
    Illegal BglII site found at 24215
    Illegal BglII site found at 26772
    Illegal BamHI site found at 7510
    Illegal BamHI site found at 13032
    Illegal BamHI site found at 18279
    Illegal BamHI site found at 27709
    Illegal XhoI site found at 1749
    Illegal XhoI site found at 3005
    Illegal XhoI site found at 7764
    Illegal XhoI site found at 13286
    Illegal XhoI site found at 18533
    Illegal XhoI site found at 23050
    Illegal XhoI site found at 27963
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7794
    Illegal EcoRI site found at 13316
    Illegal EcoRI site found at 18563
    Illegal EcoRI site found at 27993
    Illegal XbaI site found at 14126
    Illegal XbaI site found at 19373
    Illegal XbaI site found at 28803
    Illegal PstI site found at 1920
    Illegal PstI site found at 3161
    Illegal PstI site found at 5716
    Illegal PstI site found at 7543
    Illegal PstI site found at 8683
    Illegal PstI site found at 11238
    Illegal PstI site found at 13065
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7794
    Illegal EcoRI site found at 13316
    Illegal EcoRI site found at 18563
    Illegal EcoRI site found at 27993
    Illegal XbaI site found at 14126
    Illegal XbaI site found at 19373
    Illegal XbaI site found at 28803
    Illegal PstI site found at 1920
    Illegal PstI site found at 3161
    Illegal PstI site found at 5716
    Illegal PstI site found at 7543
    Illegal PstI site found at 8683
    Illegal PstI site found at 11238
    Illegal PstI site found at 13065
    Illegal AgeI site found at 7872
    Illegal AgeI site found at 13394
    Illegal AgeI site found at 18641
    Illegal AgeI site found at 28071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization, gene copy number control


Source

Commercial synthetic

References